Optimization of the enzymatic extraction of oil from the seeds of vutalao (Calophyllum inophyllum L.)

Issue Date

8-2009

Abstract

Enzymatic extraction process was utilized to recover oil from the seeds of vutalao (Calophyllum inophyllum L.). Optimum enzyme mix of three crude enzymes (xylanase, a-amylase and cellulase) was used to determine the optimum condition in terms of temperature (40, 50 and 60˚C), incubation time (1, 3, and 5 h), and enzyme concentration (1.5, 2 and 2.5%). Optimization was conducted using Response Surface Methodology employing three-level Box-Behnken design. Results showed that the maximum oil recovery at 96.99% occurred at 47.17˚C, with an incubation time of 3.56 h and enzyme concentration of 2.08% (by weight). Furthermore, oil recovery increased from 40˚C up to the optimum value then decreased when the temperature was further increased (> 48˚C), whereas, oil recovery increased when the incubation time (1.3 to 5 h) and enzyme concentrate (1.6 to 2.5%) were increased.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

34

Issue

2

Page

38-47

Document Type

Article

Frequency

tri-quarterly

Physical Description

tables, chart, graphs

Language

English

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