Morphological, molecular, cytogenetic and agronomic evaluations of rice (Oryza sative L.) mutants cv. NSIC Rcl44

Issue Date

12-2016

Abstract

Using induced mutation in any breeding program requires a thorough verification of the authenticity of the derived mutants. Field evaluation of the promising lines is useful to assess stability of its agronomic performance. In this study, 10 mutant lines together with the wild type NSIC Rc144 were characterized using phenotypic, molecular and cytogenetic traits. Field evaluation was set up in two seasons (wet and dry) following Randomized Complete Block Design using two check cultivars (PSB Rc 82 and NSIC Rc 144). Result of morphological characterization showed similarity as well as deviation in some traits of mutants from its original cultivar. Molecular characterization using 39 SSR markers revealed mutation-induced polymorphism. Cluster analysis using morphological data in comparison with molecular data revealed authenticity of the mutants as derived from NSIC Rc 144. It further displayed that molecular approach appeared effective than morphological approach since there is less ambiguity compared to the phenotypic data analyzed. Cytogenetic evaluation did not give much information in terms of changes in chromosome configuration of the mutants. Field evaluation revealed significantly higher performance of the 2 mutants for it out-yielded the wild type and the check cultivars.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463X

Volume

41

Issue

3

Page

30-39

Document Type

Article

Physical Description

illustrations; graphs; tables

Language

English

Subject

Oryza sativa L.; Rice varieties; Food consumption

En – AGROVOC descriptors

ORYZA SATIVA; RICE; VARIETIES; MUTANTS; FIELDS; EVALUATION; AGRONOMIC CHARACTERS; CYTOGENETICS; MOLECULAR GENETICS; GENETIC MARKERS

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