Genetic variability of Ralstonia solanacearum (E.F. Smith) Yabuuchi et al. isolated from soil planted to different solanaceous crops in the Philippines

Issue Date

4-2015

Abstract

Ralstonia solanacearum ( E. F. Smith) Yabuuchi et al. isolated from soil planted to different solanaceous crops were characterized based on traditional methods such as cultural characterization and pathogenicity test. Isolate were collected from different areas in Luzon, specifically in Beguet and areas in Mindanao (Misamis Oriental and Bukidnon) [Philippines]. Using the species-specific primers 759f/760r in multiplex-polymerase chain reaction, a 281-bp amplicon was generated from all the isolates which further confirm the identity of the bacterial isolates as R. solanacearum. Moreover, multiplex-PCR using phylotype-specific primers revealed that the Mindanao isolates belong to phylotype 1 while the Benguet isolates belong to phylotype 2. Assessment of the genetic diversity of the R. solanacearum isolates by repetitive element (rep)-PCR using REP and BOX primers showed the separation of the Mindanao isolates from the Benguet isolates based on geographic origin. These characterization studies are vital for analyzing the difference between the isolates affecting the solanaceous crops in the 'vegetable baskets' of Luzon and Mindanao which can be used for studies related to disease management, host resistance and genetic relationships.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463X

Volume

40

Issue

1

Page

45-53

Document Type

Article

Physical Description

illustrations; tables

Language

English

Subject

Ralstonia solanacearum; Bacterial wilt diseases

En – AGROVOC descriptors

RALSTONIA SOLANACEARUM; GENETIC VARIATION; WILTS; PATHOGENS; PATHOGENICITY; DISEASE CONTROL; CROP MANAGEMENT; PHILIPPINES

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