Randomly amplified polymorphic DNA (RAPD) analysis to reveal genetic relationship among pineapple (Ananas comosus (L.) Merr.) genotypes

Issue Date

12-2009

Abstract

The identity of various clone of the major pineapple varieties such as 'Smooth Cayenne', 'Queen' and 'Red Spanish' is not clear. The genetic diversity of 22 genotype was analyzed using randomly amplified polymorphic DNA (RAPD). Sixty-two decamer oligonucleotide Operon primers were screened for polymerase chain reaction (PCR) band polymorphism among the genotypes. Five of these primers, namely: OPS-12, OPY-15, OPAV-03, OPAV-06 and OPAK-19 yielded consistent amplification in repeated trials, and were subsequently used in the diversity analysis. The five primers amplified a total of 40 distinct fragments, 85% of which were polymorphic. Each primer produced 5-13 fragments with sizes between 0.40 2.36 kb. The pineapple genotypes were scored for the presence or absence of the RAPD fragments and were grouped by cluster analysis based on simple matching coefficients of similarity. A dendrogram of the 22 genotypes separated the pineapple cultivars from their wild relatives. These cultivars clustered into four major groups namely: i) 'Red Spanish' and its hybrids, ii) 'Smooth Cayenne' cultivars, iii) hybrids of 'Smooth Cayenne' and Ananas lucidus Miller belonged to a distinct group. The genetic diversity of the 19 Ananas comosus (L) Merr. cultivars used is narrow as indicated by the high similarity coefficient, but this becomes wider with the addition of the wild relatives. The result implies practically that A. comosus could have originated from one genotype but due to human selection, useful clones were isolated and evolved into a variety. It is suggested that in order to enhance the genetic diversity of pineapple, mutation breeding and induction of somacional variation could be done. It is recommended to pursue wide hybridization by crossing A. comosus with related species (e.g A. lucidus, A. bracteatus, A. ananassoides) to increase the genetic diversity of the pineapple cultivars.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

34

Issue

3

Page

1-10

Document Type

Article

Frequency

tri-quarterly

Physical Description

RAPD band profile

Language

English

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