Morphometrics of hepatic and splenic melanomacrophage centers (MMCS) of nile tilapia (oreochromis niloticus Linn.) after exposure to imidacloprid

Issue Date

10-2014

Abstract

The potential of melanomacrophage centers (MMCs) found on Nile tilapia (Oreochromis niloticus, Linnaeus) as precise biomarker of fish and environmental health was evaluated. The morphometrics (count, size, and pigments composition) of hepatic and splenic MMCs were assessed after exposure to imidacloprid insecticide. Fish were exposed to sublethal concentrations (0,0.013,0.067 and o.134 mg/L) of imidacloprid and disseted at 0, 7, 14, and 21 days. Results showed that exposure to sublethal concentrations of imidacloprid caused significant changes (P<0.05) in splenic MMC count among test fish. The development of splenic MMCs showed an increasing trend after 21 days of exposure to imidacloprid insecticide. On the other hand, there were no significant differences found in splenic MMC size, hepatic MMC count and size in treatment groups as compared to the reference group. Analysis revealed that the pigment composition of MMCson both liver and spleen tissue samples was comprised of lipofuscin and hemosiderin. Between the two pigments, there were significant differences observed (P<0.05) in lipofuscin content of hepatic MMCs between the untreated fish and those fish exposed to imidacloprid. Hence, this study determined that MMCs were sensitive and effective biomarker of stress as indicated by changes in splenic MMC count and proliferation of lipofuscin.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

28

Issue

2

Page

223-224

Document Type

Article

Frequency

semi-annually

Language

English

En – AGROVOC descriptors

OREOCHROMIS NILOTICUS; MARCHANTIOPHYTA; IMIDACLOPRID; INSECTICIDES; INDICATOR ORGANISMS; GENETIC MARKERS; LIVER

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