Detecting hybrid admixtures through DNA analysis

Issue Date

4-2012

Abstract

This paper discusses the methodologies and application of DNA-based protocol for detecting hybrid admixtures in seed lots. Using microsatellite DNA markers, we successfully detected hybrid admixtures in Certified Seeds of 'Mestizo 1', 'Mestiso 3', and 'Mestiso 7'. Four Certified Seed lots of hybrid seeds were analyzed through DNA-based after seed purity using markers RM110, RM234, RM171, and RM263. DNA fingerprints were detected in 8% non-denaturing polyacrylamide gels and ethidium bromide staining. Hybrid admixtures of seed lots from NSQCS Isabela were 6.4% and 10.9% for 'Mestizo 1' and 'Mestiso 7', respectively. Seeds obtained from NSQC Davao showed 6.4% and 3.3% in Mestiso 3 and Mestiso 7, respectively. Contrary to results obtained using conventional methods, our data show that DNA-based methods are capable of differentiating true-to-type hybrids from non-hybrids.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

37

Issue

1

Page

42-46

Document Type

Article

Frequency

tri-quarterly

Physical Description

tables, graph, pictures

Language

English

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