Optimization of DNA extraction protocol for Hoya mindorensis Schlechter

Issue Date

8-2011

Abstract

Hoyas are popular ornamental plants originating from Southeast Asia. No publication on DNA extraction protocol on any Hoya genus is available. Several protocols were tested for suitability in DNA extraction of Hoya mindorensis Schlechter. A modified Angeles et al. (2005) protocol has been developed and proved to be successful in obtaining DNA products. This protocol utilized high-salt extraction buffer (2.0 M NaCl). The amount of b- mercaptoethanol concentration was modified to 0.5 M and the use of SDS was omitted. The DNA obtained through this protocol was amplified successfully through PCR and was able to produce distinct polymorphic SSR bands.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

36

Issue

2

Page

63-66

Document Type

Article

Frequency

tri-quarterly

Physical Description

table, DNA extractions

Language

English

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