Issue Date
10-2013
Abstract
Brush border membrane vesicles (BBMVs) were prepared from 4th instar larvae of Asian corn borer Ostrinia furnacalis Guenee. SDS-PAGE analysis showed major protein bands with molecular weight in the range of 80 kDa to 250 kDa. Ligand blot analysis showed that biotinylated Cry1Ab δ-endotoxin strongly binds to a ~250 kDa receptor protein from 0. furnacalis BBMV. Another receptor protein of ~110 kDa weakly binds to Cry1Ab δ-endotoxin. At least two isoforms of the ~250 kDa receptor protein have a pH range of 5.5 to 6.5 as determined by 2D-electrophoresis. Post-translational modification of 0. furnacalis BBMV proteins was also detected as revealed by the presence of two glycosylated proteins with molecular weights of >250 kDa and 70 kDa, respectively. Alanine scanning mutations were introduced in domain III ß-sheet 13 of Cry1Ab δ-endotoxin. Results of bioassays showed a 5 to 7 fold reduction in insecticidal activity in Serine556Alanine, Serine557Alanine and Serine556Alanine-Serine557Alanine mutants of Cry1Ab protein. The decrease in insecticidal activity suggests that Serine556 and Serine557 are both important residues in the toxicity of Cry1Ab δ-endotoxin to 0. furnacalis.
Source or Periodical Title
The Philippine Entomologist
ISSN
0048-3753
Volume
27
Issue
2
Page
120-133
Document Type
Article
Frequency
semi-annually
Physical Description
illustrations ; tables
Language
English
Recommended Citation
Alcantara, Edwin P. and Bayot, Aileen N., "Biochemical characterization of midgut receptor Asian corn borer Ostriniafurnacalis Guenee (Lepidoptera: Pyralidae) and modification of domain III ß-sheet 13 of Bacillus thuringiensis Cry1Ab δ-endotoxin" (2013). Journal Article. 4780.
https://www.ukdr.uplb.edu.ph/journal-articles/4780
En – AGROVOC descriptors
OSTRINIA FURNACALIS; BACILLUS THURINGIENSIS; ALANINE; ENDOTOXINS; PESTS OF PLANTS; BACTERIAL PESTICIDES; RECEPTORS