Diversity and host range of entomopathogenic nematodes isolated in the Philippines

Issue Date

10-2013

Abstract

From a total of 88 location s in the Philippines, seven soil samples, which included soils from Batangas, Bukidnon, Laguna and South Cotabato, were positive for the presence of entomopathogenic nematodes (EPN). These collection sites were planted to any of the following crops: bignay, cassava, corn, mango or sugarcane. The EPNs were collected through the insect-baiting technique, purified and maintained in vivo using live Asian corn borer, Ostrinia furnacalis, larvae as baits. Preliminary morphological and molecular characterization based on the amplified ITS-5.8S rDNA region revealed six EPN strains belonging to Steinernemaspp., while the other was identified as Heterorhabditis sp. Host range studied of all the EPN isolates confirmed their pathogenicity against corn earworm Helicoverpaarmigera, the common cutworm Spodoptera litura and the armyworm Mythimna separate. Optimization of bioassay conditions is still underway to determine the best species for development as biological control agent.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

27

Issue

2

Page

200

Document Type

Article

Frequency

semi-annually

Language

English

En – AGROVOC descriptors

OSTRINIA FURNACALIS; ENTOMOPHILIC NEMATODES; STEINERNEMA; PESTS OF PLANTS; PEST CONTROL; PHILIPPINES

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