Genome-wide associations and transcriptional profiling reveal ROS regulation as one underlying mechanism of sheath blight resistance in rice

Issue Date

12-2019

Abstract

Rice sheath blight, caused by the necrotrophic fungus Rhizoctonia solani Kühn, continues to be an important and challenging rice disease worldwide. Here, we used genome-wide association studies over a high-density rice array to facilitate the identification of potential novel genes and quantitative trait loci related to sheath blight resistance. We identified multiple regions that significantly associated with independent disease components in chromosomes 1, 4, and 11 under controlled condition. In particular, we investigated qLN1128, a quantitative trait locus enriched with defense-related genes that reduce disease lesions in a near-isogenic line. RNA profiling of the line carrying qLN1128 showed a number of differentially expressed genes related to the reactive oxygen species (ROS)-redox pathway. Histochemical staining revealed less ROS accumulation on the resistant line, suggesting efficient ROS deregulation that delays pathogen colonization. The detection of genomic regions controlling multiple mechanisms of resistance to sheath blight will provide tools to design effective breeding interventions in rice.

Source or Periodical Title

Molecular Plant-Microbe Interactions

ISSN

0894-0282

Volume

33

Issue

2

Page

212-222

Document Type

Article

Physical Description

illustration, charts, graphs, table, references

Language

English

Subject

Complex disease, Genomewide association study (GWAS), Quantitative trait loci (QTLs), Reactive oxygen species (ROS), Rhizoctonia solani, Rice sheath blight

Identifier

doi: 10.1094/MPMI-05-19-0141-R.

Digital Copy

Yes

En – AGROVOC descriptors

COMPLEX DISEASE; GENOMEWIDE ASSOCIATION STUDY (GWAS); QUANTITATIVE TRAIT LOCI (QTLs); REACTIVE OXYGEN SPECIES (ROS); RHIZOCTONIA SOLANI; RICE SHEATH BLIGHT

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