Inheritance, stability and mechanism of fenvalerate resistance in the red flour beetle, Tribolium castaneum Herbst

Issue Date

4-2012

Abstract

Fenvalerate resistance in the red flour beetle (Tribolium castaneum Herbst) was characterized using fernvalerate-selected (R-fen1) strain. Results of crossing experiments between the resistant and susceptible strains showed that resistance id multifactorial and the inheritance incompletely dominant. In the absence of selection pressure, fernvalerate resistance was stable for 6 (ca. 6 months) generations, and then partially declined during the succeeding 8 generations. Judging from the KT50 values obtained by topical application of fenvalerate against larvae, R-fen1 strain showe 2.2 fold knockdown resistance (kdr) as compared to the susceptible counterpart. The pretreatment with the metabolic inhibitors PBO and DEF shortened the knockdown time to some extent in fenvalerate-resistant strain but not to the level of the susceptible strain, indicating again the presence of kdr factor in the resistant strain. The resistant strain exhibited two to three times larger cytochrome P450 content than that in the susceptible strain as estimated by the CO-binding difference spectrum in both larvae and adults. In vitro comparison of microsomal degradation of 14C-fenvalerate between R-fen1 and susceptible strains in the presence of NADPH demonstrated an increased degradation activity by the cytochrome P450-dependent monooxygenase system as additional factor for fenvalerate resistance.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

26

Issue

1

Page

1-12

Document Type

Article

Frequency

semi-annually

Physical Description

illustrations ; tables

Language

English

En – AGROVOC descriptors

TRIBOLIUM CASTANEUM; FENVALERATE; STORED PRODUCT PESTS; INSECTICIDES; DEFENSE MECHANISMS; INSECTICIDE RESISTANCE

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