Optimization of the nucleopolyhedrovirus SpltMNVP-P7 production in its homologous host, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)
Issue Date
4-2021
Abstract
An efficient mass production protocol was developed for the Philippine strain of Spodoptera litura nucleopolyhedrovirus, SpltMNPV-P7. A three-step study was designed to determine the larval age, amount of inoculum and post-infection harvest time that would efficiently yield the highest number of occlusion bodies (OBs) in S. litura larvae. The highest number of OB produced was observed in 9 days old larvae (2.667 x 107 ± 0.602 x 107), indicating that 9 days is the most ideal age of the host larvae for SpltMNPV-P7 mass production. Among the four inoculum concentrations used to infect 9 days old larvae, 10,000 OBs/larva produced the highest yield of OBs (4.167 x 108 ± 0.361 x 108), thus, the combination of 9 days old larvae and 10,000 OBs/larva was used to determine the optimum post infection period for harvesting SpltMNPV-P7, which was found to be 6 days post-infection (DPI) that gave the highest OB yield (7.004 x 109 ± 1.513 x 109). Thus, the best larval age, inoculum concentration and post-infection harvest time for optimum OB yield of SpltMNPV-P7 in S. litura are 9 days old, 10,000 OBs/larva and 6 DPI, respectively.
Source or Periodical Title
The Philippine Entomologist
ISSN
0048-3753
Volume
26
Issue
1
Page
54-64
Document Type
Article
Frequency
semi-annually
Physical Description
illustrations; tables
Language
English
Recommended Citation
Velasco, Aileen Gail V. and Caoili, Barbara L., "Optimization of the nucleopolyhedrovirus SpltMNVP-P7 production in its homologous host, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)" (2021). Journal Article. 4839.
https://www.ukdr.uplb.edu.ph/journal-articles/4839
En – AGROVOC descriptors
SPODOPTERA LITURA; LARVAE; ANIMAL DEVELOPMENT STAGE; NUCLEAR POLYHEDROSIS VIRUS; INFESTATION; MASS REARING; IN VIVO EXPERIMENTATION
