Preliminary study on the mitochondrial DNA Cytochrome C Oxidase Subunit II gene of the African armyworm Spodoptera exempta Walker (Lepidoptera: Noctuidae) in the Philippines

Issue Date

10-2012

Abstract

Molecular analysis on the mitochondrial DNA cytochrome oxidase c subunit II gene (mtDNA COII) of Spodoptera exempta was done to determine any variation within the Sariaya (SRY1, SRY5a, SRY6a and SRY9b) and between the Sariaya and Lipa (LIPA) populations. The partial COII gene from fifth instar larvae of S. exempta was amplified using COII-specific primers (Hoshizaki et al., 2008). Variations in nucleotide and amino acid sequences of the COII gene were observed amongst the S. exempta samples from the two localities. Multiple sequence alignments of the nucleotide sequences identified the sources of variations, namely: transition, transversion and deletion, between and within samples from the two localities. The phylogram showed genetic distance relationships among S. exempta samples, S. exigua, S. frugiperda, Bombyx mori and Hyphantria cunea. This preliminary study demonstrates the potential of the mtDNA COII gene as a source of biological information, which can be used in phylogeographical studies of not only S. exempta but also other species for monitoring and designing pest control strategies for insect pest species in the Philippines.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

26

Issue

2

Page

120-136

Document Type

Article

Frequency

semi-annually

Physical Description

illustrations ; tables

Language

English

En – AGROVOC descriptors

SPODOPTERA EXEMPTA; MITOCHONDRIAL DNA; CYTOCHROME C OXIDASE; GENETIC MARKERS; PHILIPPINES

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