Characterization of the QTL linked to tungro and green leafhopper resistance in rice (Oryza sativa L.) using advance backcross population

Issue Date

8-2016

Abstract

Resistance to rice tungro spherical virus (RTSV) and green leafhopper (GLH) found in the Indian landrace, ARC11554, was previously localized on the short arm of rice chromosome 4 flanked by markers RM8213 and RM3471. This study aimed to infer the haplotype of the introgression region in backcross lines, determine the backcross line containing the resistance region that is most suitable for developing a mapping population and narrow down the region of the putative chromosomal position of the resistance QTL using tightly linked molecular markers in an F₂ mapping population. Fifty-five backcross lines (11 BC₂F₆ and 44 BC₄F₄) in NSIC Rc138 background were used for haplotype characterization. Of the 55 lines, 22 backcross lines that resembled the donor region in chromosome 4 were selected for marker similarity analysis. Marker similarity ranged 81-86% among the BC₂F₆ and 89-99 among BC₄F₄ lines. Mapping population composed of 1,014 BC₅F₂ was established from the cross between NSIC Rc138 and ARC138-4-5-6-2-30. The BC₅F₂:₃ lines were screened for tungro and GLH resistance. QTL detected for RTSV resistant accounted for 17.43% of the total phenotypic variance explained (PVE) and the GLH resistance QTL had a PVE of 19.19%. Two peaks were observed: one on the left side of RM8213 (RM335-RM16428) and a secondary peak on the right (RM16434-RM16497). The primary signal (peak LOD) was located in the RM16425-RM16427 region, the size of which is only 0.5 kb.. This confirmed that the very strong resistance of ARC11554 is due to cosegregation of resistance genes conferring resistance to RTSV and GLH.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

41

Issue

2

Page

20-30

Document Type

Article

Frequency

tri-quarterly

Physical Description

tables,dendrograms, graphs

Language

English

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