Natural medium for growing endophytic bacteria from solanaceae

Issue Date

6-2016

Abstract

Endophytic bacteria are important microorganism that are potential biocontrol agents for many pathogens. It growth requires the use of semisynthetic or synthetic medium the ingredients of which make it difficult for farmers to use in the field. It is also expensive for propagation as biocontrol agents. The study focused on identifying natural ingredients as medium for growing the endophytic bacteria. The medium developed have natural ingredients like soybean, chicken broth, egg, worms, snail, and sorghum, which are easy to obtain by farmers. The factors were arranged in completely randomized factorial design for four isolates of endophytic bacteria from solanaceae in Malang, Indonesia, and five natural ingredients for the the medium-corn flour, soybean flour, sorghum flour, snail flour, and worm flour. Result of the study showed that the bacteria grew in all of the media used. There is no difference in the incubation period, color, shape, and surface colony. However, the population in the snail flour medium at 10⁷ cfu mLˉ¹ is the highest ( 7.5x10⁶ cfu mLˉ¹ ), thus, the best medium for growing endophytic bacteria.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

41

Issue

1

Page

26-29

Document Type

Article

Frequency

tri-quarterly

Physical Description

picture, graphs

Language

English

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