Genetic diversity and geographic dispersion in Thymus spp, as detected by RAPD markers

Issue Date

4-2015

Abstract

Thyme, as an aromatic medicinal plant and a perennial and woody herb from Lamiaceae has commercial, pharmaceutical and perfumery potentialities. Thymus is taxonomically a very complex genus with a high frequency of hybrid of hybridization and introgression among sympatric species, and some species of this herb are endemic to Iran. From the chemical point of view, important biochemical components such as thymol and carvacrol are known in thyme. In the present study, 13 Thymus spp. accessions collected from different geographic areas of Iran along with one accession from England (Thymus vulgaris) were analyze by Random Amplified Polymorphic DNA (RAPD) markers using 20 primers to discover genetic polymorphism. A total number of 510 bands were detected from 20 RAPD primers, of which 483 (94.31%) were polymorphic, with an average of 24.15 polymorphic bands per primer. The size range of the amplified products was 200-4000 bp. UPGMA cluster analysis was carried out using Jaccard similarity coefficients based on RAPDs. The dendrogram obtained from the method classified the 14 thyme accession into four major groups. Scatter biplot based on principal coordinate analysis (PCoA) also revealed four groups and confirmed the results of clustering method with some minor disagreements. The accession were relatively grouped according to the location where they had been collected. The molecular variation assessed to the study could elucidate largely geographic dispersion of the thyme accessions, and in combination with biochemical characteristic, can be useful to improve the efficiency of selection and breeding programs.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

40

Issue

1

Page

82-88

Document Type

Article

Frequency

tri-quarterly

Physical Description

tables, dendrograms

Language

English

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