Enhanced organic phosphate utilization by over-expression of OsACP1 and OsPAP1 genes in rice (Oryza sativa L.)

Issue Date

4-2015

Abstract

Phosphorus is one of the most important macro-elements for plant growth and development. It can easily combine with other minerals, however, when converted into insoluble form, phosphate may not be available for plant use. Among insoluble phosphates, organic phosphate (Po) accounts for 20-80% of the total phosphate in soil. Po should be hydrolyzed by phosphatases so that it can be absorbed by plants. OsACP1 (gene coding phosphatase isolated from Oryza sp.) and OsPAP1 (O. sativa purple acid phosphates 1) genes (gene coding phosphatase isolated from Oryza sp. have been studied in rice, while their function has not been fully investigated. In this study, transgenic rice, OsACP1-OX1, and OsPAP1-OX, were generated by Agrobacterium-mediated transformation in the Japonica rice cultivar Dongjinbyeo to determine the utilization and uptake of Po by over-expression of endogenous OsACP1 and OsPAP1 genes. Transgenic lines were selected based on the OsACP1 and OsPAP1 gene expression. Homozyous plants of the T3 generation were used. Transgenic plants with over-expression of OsACP1 and OsPAP1 showed higher phosphatase activity than the wild type. Po is then hydrolyzed such that more Pi is absorbed in the transgenic plants than the wild type. Plant height and tiller number were increased by 120-200% in the transgenic plants. These results indicate that OsACP1 and OsPAP1 could enhance the organic phosphate uptake and utilization in rice and ultimately increase efficiency in phosphate use and increase productivity.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

40

Issue

1

Page

17-23

Document Type

Article

Frequency

tri-quarterly

Physical Description

picture, graphs

Language

English

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