Identification of tightly-linked markers for the genic male sterile gene of ms-IR36 in rice (Oryza sativa L.)

Issue Date

4-2015

Abstract

Recurrent selection is being adopted by rice breeders to broaden the genetic diversity of the breeding populations, quickly assemble desirable alleles from multiple sources and increase the chance of obtaining outstanding recombinants. Nuclear male sterile genes can be utilized in recurrent selection to facilitate intermating. A male sterile mutant of the popular variety IR 36, ms-IR36 is used in most recurrent selection programs in rice to facilitate intermating and eliminate hand emasculation. The male sterility of was conferred by a recessive nuclear gene, designated as ms. In this study, selective genotyping applied to an ms-IR36/Jamaica 3 F₂ population located the ms gene onto the short arm of chromosome 2. Using 565 male sterile plant from selected F₃ families of ms-IR36/Zileng 8-3, the ms gene was fine mapped to a 28-kb region between SSSR markers, RM12351 and RM12354 with genetic distance of 0..8 and 0.09 cM. Six markers tightly linked to the ms gene were used in the development of new male sterile lines through backcrossing. Moreover, Tightly linked markers can be used in selecting homozygous fertile plants from from recurrent selection programs.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

40

Issue

1

Page

1-7

Document Type

Article

Frequency

tri-quarterly

Physical Description

picture, tables

Language

English

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