Isolation and characterization of Aspergillus niger TR-H degrading the chlorimuron-ethyl herbicide

Issue Date

4-2013

Abstract

A fungal strain, named TR-H capable of degrading the herbicide chlorimuron-ethyl, 2-([(4-chloro-6-methoxypyrimidine-2-yl) amino carbonyl] benzoic acid, ethyl ester was isolated from a chlorimuron-ethyl polluted soil and identified as Aspergillus riger based on morphological characteristic and 18S rRNA gene sequence data. Incubation tests showed that when the initial concentration of Chlorimuron-ethyl in culture was 10.0 mg L¯¹, TR-H could degrade 96.59% of the herbicide chlorimuron-ethyl after incubation for 7 d at 30˚C. HPLC-MS (High Performance Liquid Chromatography-Mass Spectrometry) method were employed to define degradation products and metabolic pathway of chlorimuron-ethyl.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

38

Issue

1

Page

52-56

Document Type

Article

Frequency

tri-quarterly

Physical Description

pictures, charts, graphs

Language

English

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