In vitro root and shoot formation of Rosa centifolia using plant growth regulators
Issue Date
8-2018
Abstract
Rosa centifolia is one of the most economically important floriculture crops that is grown and used for oil extract which has various applications in pharmaceutical industries. It is commercially propagated through cuttings, but low rooting percentage has always been a problem since rose species are difficult to root. To address this problem, a protocol was developed to meet the growing demands for R. centifolia through in vitro propagation technique. Nodal segments were used as an explant and were cultured in a MS medium supplemented with different concentrations (0.2, 0.4, 0.6 and 0.8μM) of benzyllaminopurine (BAP) and Kinetin (KIN), alone and in combination of both, for induction of shoots. For root induction,different concentrations (0.2, 0.4, and 0.6μM) of Indole Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA) were used in a ½ strength MS medium. BAP at 0.2μM concentration produced the highest number of shoots (1.93) and longest shoot elongation (5.55cm) vis-a-vis other concentrations. Moreover, minimum number of days (10.2) to shoot induction was observed at 0.4μM of BAP in combination with 0.2μM of KIN. On the other hand, IBA at 0.4μM had the minimum number of days (10.07) to produce roots, highest number of roots (2.47), and longest root elongation (4.21cm) among other concentrations. Thus, it is concluded that BAP at 0.2μM and IBA at 0.4μM concentration are effective for shoot and root formation of R. centifolia, respectively.
Source or Periodical Title
Philippine Journal of Crop Science
ISSN
0115-463x
Volume
43
Issue
2
Page
63-70
Document Type
Article
Frequency
tri-quarterly
Physical Description
tables, pictures
Language
English
Recommended Citation
Akhtar, Gulzar; Jaskani, Muhammad Jafar; Sajjad, Yasar; Akram, Ahsan; Farooq, Amjad; and Rasool, Ghulam, "In vitro root and shoot formation of Rosa centifolia using plant growth regulators" (2018). Journal Article. 5136.
https://www.ukdr.uplb.edu.ph/journal-articles/5136