Optimization of in vivo mass-production of nucleopolyhedrovirus, SpltMNPV-P7, infecting the common cutworm, Spodoptera litura Fabr.

Issue Date

10-2011

Abstract

The larval age of Spodoptera litura that would give the highest virus yield for SpltMNPV-P7 was determined. S. litura larvae aged 6-days old, 9 days old and 1-day-old S. litura larvae were treated with 100 OBs/larva. The gain in weight was monitored at 2-,4- and 6-days post infection (DPI). OBs were harvested from the infected larvae at 6 days post-infection and counted with an improved Neubauer Hemacytometer. The highest weight gain and OB production at 6 DPI were observed in 9-days old larvae. In contrast, the lowest OB yield and average larval weight gain were recorded 6-days old larvae. The 12-days old larvae were inefficient hosts for in vivo mass-production SpltMNPV-P7 since almost all larvae had pupated at the time of harvest. These results show that among the three larval ages, 9-days is the most ideal larval age for NPV mass-production since the insects achieved their optimum larval weight gain and produced the most number of OBs at a shorter time.

Source or Periodical Title

Philippine Entomologist

ISSN

0048-3753

Volume

25

Issue

2

Page

205

Document Type

Article

Frequency

semi-annually

Language

English

En – AGROVOC descriptors

SPODOPTERA LITURA; INSECTA; IN VIVO DIGESTIBILITY; WEIGHT GAIN; INFECTION

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