Optimization of in vivo mass-production of nucleopolyhedrovirus, SpltMNPV-P7, infecting the common cutworm, Spodoptera litura Fabr.
Issue Date
10-2011
Abstract
The larval age of Spodoptera litura that would give the highest virus yield for SpltMNPV-P7 was determined. S. litura larvae aged 6-days old, 9 days old and 1-day-old S. litura larvae were treated with 100 OBs/larva. The gain in weight was monitored at 2-,4- and 6-days post infection (DPI). OBs were harvested from the infected larvae at 6 days post-infection and counted with an improved Neubauer Hemacytometer. The highest weight gain and OB production at 6 DPI were observed in 9-days old larvae. In contrast, the lowest OB yield and average larval weight gain were recorded 6-days old larvae. The 12-days old larvae were inefficient hosts for in vivo mass-production SpltMNPV-P7 since almost all larvae had pupated at the time of harvest. These results show that among the three larval ages, 9-days is the most ideal larval age for NPV mass-production since the insects achieved their optimum larval weight gain and produced the most number of OBs at a shorter time.
Source or Periodical Title
Philippine Entomologist
ISSN
0048-3753
Volume
25
Issue
2
Page
205
Document Type
Article
Frequency
semi-annually
Language
English
Recommended Citation
Velasco, Aileen Gale V. and Caoili, Barbara L., "Optimization of in vivo mass-production of nucleopolyhedrovirus, SpltMNPV-P7, infecting the common cutworm, Spodoptera litura Fabr." (2011). Journal Article. 5218.
https://www.ukdr.uplb.edu.ph/journal-articles/5218
En – AGROVOC descriptors
SPODOPTERA LITURA; INSECTA; IN VIVO DIGESTIBILITY; WEIGHT GAIN; INFECTION