Isohemoglobins of the Milkfish, Chanos chanos (Forskall) (Osteichthyes: Chanidae) Collected from two sites in Laguna de Bay

Issue Date

6-1990

Abstract

Blood samples from 56 ten-month-old milkfish Chanos chanos (forskall) were analyzed using polyacrylamide disc gel electrophoresis (PAGE). Twenty-eight fish were collected from fishpens in each site : Calamba, Laguna (Site A) and Alabang, Metro Manila (Site B).

A total of 23 isohemoglobins were observed from all the samples examined. Multiple isohemoglobins in complex and heterogeneous pattern ranging from 5-13 in Site A and 5-14 in Site B were manifested. Isohemoglobin 1, the fastest moving band which is uniquely expressed in Site B, has an average Rf of 0.984 while isohemoglobin 23, the slowest moving band found in both sites, had an average Rf of 0.138. Cathodal isohemoglobin were predominantly expressed in both sites while anodal isohemoglobins occurred in higher frequency in Site B that in Site A.

Statistical analysis for equality of variance of the relative mobility of isohemoglobins revealed that there is a significant difference in the variability of isohemoglobins between the two sites at 1.0% level of significance. The difference in variability observe cannot, however, be correlated to the difference in oxygen content of both sites since water analysis showed that both contain roughly 7.30mg/1 of dissolved oxygen. The possible difference could be attributed to other factors in the environment, such as pH. Water in Alabang, with a lower pH than the water in Calamba, seems to favor the high incidence of anodal isohemoglobins in the former.

Source or Periodical Title

U.P. LOS BAŇOS JOURNAL

Volume

1

Issue

1

Page

70-83

Document Type

Article

Frequency

annually

Physical Description

table, photographs,

Language

English

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