Morphological characterization and ssr-based DNA fingerprinting of cassava (Manihot esculenta crantz) varieties released by the national seed industry council (NSIC)

Abstract

© 2019, College of Agriculture and Food Science, University of the Philippines Los Banos. All rights reserved. The genetic variability of 47 cassava varieties released by the National Seed Industry Council (NSIC) was evaluated through morphological characterization and DNA fingerprinting using simple sequence repeat (SSR) markers. Phenotypic similarities based on 34 morphological descriptors were observed in varieties sharing a common parent. Fifty (50) polymorphic SSR markers were used to construct DNA fingerprints. A total of 648 polymorphic alleles and 764 unique banding patterns were observed among the 47 varieties. The polymorphism information content (PIC) values ranged from 0.46 to 0.95, with an average of 0.83. Each variety had a unique banding profile indicating that SSR markers were useful in distinguishing cassava varieties. A wider genetic diversity of cassava varieties was detected in SSR data (70%) compared with morphological data (50%) showing that SSR markers are more effective in determining the extent of variation between genotypes. The DNA fingerprints of cassava varieties have been successfully generated which can be used as a benchmark for identification and authentication of released cassava varieties.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

347-357

Document Type

Article

Subject

Cassava, DNA fingerprinting, National Seed Industry Council, PIC, SSR

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