Development of genomics tools to assist breeding of mango for desired traits, hybridity testing, and barcoding for diversity analysis and variety identification

Issue Date

6-2017

Abstract

Carabao is the most popular mango variety for the domestic and export market. However, its production is besetted with many problems leading to large losses during preharvest and postharvest stages. Breeding for better Carabao mangoes with desirable traits can be assisted well with the use of molecular tools that can identify genotypes, and even gene variants for desired functional and horticultural traits. Another challenge is the polyembryonic nature of Carabao mangoes that results in multiple seedlings in one fertilized seed; hence identification of the true hybrid is difficult. Germplasm collections of mango varieties, cultivars and strains in the country need to be characterized phenotypically and genetically in order to remove duplication, identify unique genotypes, measure diversity and identify sources of important genes. Separable and previous researches have already identified mangoes that have thick peel, anthracnose and fruit fly resistance, and red blush. Efforts are underway to introduce to Carabao varieties these traits through conventional breeding. To help identify true hybrid seedlings, polymorphic molecular markers for the parental have been identified, and hybridity testing of 35 putative htbrids has commenced. Moreover, genetic barcoding for mango was initiated, with the identification of the best barcoding markers among were matk, rbcL, ITS, and trnf. In addition, DNA samples of 188 genotypes were sent for Genotyping by Sequencing (GBS) to acquire a reduced genome of the sample that will be aligned to markers/genes in NCBI BLASTn database for nucleotide sequence alignment, identification, and SWISS-Prot database for protein-related sequences. The GBS data will also be used for identifying markers such as SNPs and SSRs, and for association of selected traits with genotypes. More DNA is being prepared to add to our stock of genetic data.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463X

Volume

42

Issue

Supplement no. 1

Page

97-98

Document Type

Article

Language

English

Subject

Mango; Plant breeding

En – AGROVOC descriptors

MANGIFERA INDICA; VARIETIES; GENOMES; GENES; GERMPLASM COLLECTIONS; GENOTYPES; GENETIC MARKERS; NUCLEOTIDE SEQUENCE; BREEDING METHODS

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