Genetic diversity analysis of parental source in hybridization using microsatellite markers

Issue Date

6-2017

Abstract

Analysis of the genetic diversity of the Philippine hybridization parentals was conducted using 20 microsatellite markers. The population structure analysis was determined by Noi's Genetic Distance and Neighbor-Joining Method, and Estimated K-means Clustering. The purpose of this is to provide information for producing more focus and precise crosses that are able to maximize heterosis and hybrid performance, through determining different sub-groups within the hybridization parental and providing an estimate of genetic distance among different genotypes. Microsatellite marker data found that the hybridization parental were genetically diverse, with a 70.7% level of genetic diversity. The population showed high level of homozygosity, with 0.94 coefficient of inbreeding and a 4.14% level of heterozygosity. Analysis using K-means clustering split the 4.11 parentals into two clusters based on growing environment, with the larger cluster (350) comprised mainly of lowland varieties, while the smaller cluster (61) was comprised mainly of upland/rainfed varieties. The larger sub-cluster was further analyzed using K-means clustering to further yield four sub-clusters differentiated by the following characteristics: seedling vigor and yield; Abiotic and biotic stress resistance, micronutrient content and grain quality.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463X

Volume

42

Issue

Supplement no. 1

Page

103

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Language

English

Subject

Oryza sativa L.; Hybridization

En – AGROVOC descriptors

ORYZA SATIVA; GENETIC VARIATION; GENETIC MARKERS; GENOTYPES; HYBRIDIZATION; HETEROSIS; GENETIC DISTANCE; POPULATION STRUCTURE

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