Growth responses of in vitro-derived nodal sections of Kalanchoe blossfeldiana Poellnitz as influenced by benzylaminopurine, thidiazuron and paclobutrazol
Issue Date
7-2016
Abstract
In vitro-derived nodal explants of Kalanchoe blossfeldiana Poelin genotype 'White' were successfully regenerated in Murashige and Skoog's (1962) medium supplemented with BAP or TDZ alone or in combination with PAC. The nodal explants developed new shoots from the 7th to the 11th days after inoculation varying levels of TDZ medium, with or without PAC. The highest shoot proliferation was attained with 10 uM TDZ, producing 25 shoots by the 12th week of incubation. Addition of PAC significantly reduced the overall growth of the cultures: number of leaves, leaf width, stem diameter, internode length, shoot length and shoot proliferation. Inhibition of shoot proliferation by PAC was significantly observed when applied together with TDZ. Extensive shoot branching was observed on the 12th week after inoculation. Ethylene concentration at the headspace of the culture vessels were low in all treatments, (0.015-0.039 ppm), suggesting no influence of ethylene on the response of the K. blossfeldiana nodal cultures.
Source or Periodical Title
Asia Life Sciences
ISSN
0117-3375
Volume
25
Issue
1
Page
207-220
Document Type
Article
Frequency
semi-annually
Physical Description
tables; references
Language
English
Subject
Ornamental plants; In vitro culture; Plant propagation;
Recommended Citation
Nieves, Mylene C.; Aspuria, Emmanuel T.; and Tayangona, Maria Angie D., "Growth responses of in vitro-derived nodal sections of Kalanchoe blossfeldiana Poellnitz as influenced by benzylaminopurine, thidiazuron and paclobutrazol" (2016). Journal Article. 5632.
https://www.ukdr.uplb.edu.ph/journal-articles/5632
En – AGROVOC descriptors
KALANCHOE BLOSSFELDIANA; GROWTH; IN VITRO; BA; PACLOBUTRAZOL; THIDIAZURON; CULTURE MEDIA; GAS CHROMATOGRAPHY