Validation of newly designed SSR markers for nine rice (Oryza sativa L.) genotypes with variable heat tolerance responses based on agromorphic data and pollen fertility analysis
Issue Date
3-2023
Abstract
Rice is among the most valuable staple food crops in the world. However, several challenges greatly affect production, one of which is the threat imposed by heat stress. To address this, researchers are developing varieties that are tolerant to heat stress with the aid of genetic markers. In this study, eight rice genotypes, namely Dular, Nagina 22, NSIC Rc 222, Milyang 23, EL15, EL92, EL85, and IR52 were observed for agromorphic data, which included plant height, panicle length, filled and unfilled grains, and grain yield. Flower samples were collected to determine the effect of heat stress on pollen fertility. Molecular markers were designed via in silico analysis based on the nine QTL regions distributed among five chromosomes (1, 3, 4, 5, and 10). Out of the 90 newly developed markers, Markers 3066 and 2503 showed good potential as informative markers among heat tolerance classification. Results showed genotype-specific responses of varieties during heat stress and non-heat stress conditions. EL15 had the best agromorphic performance and appeared to be the best elite line. Identification of gene-specific SSR markers has proven to be effective in understanding heat tolerance for future marker-assisted selection.
Source or Periodical Title
Philippine Agricultural Scientist
ISSN
0031-7454
Volume
106
Issue
1
Page
75-86
Document Type
Article
Frequency
quarterly
Physical Description
illustrations, tables, graphs
Language
English
Subject
Oryza sativa, elite lines, genotypes, heat tolerance, agromorphic data, pollen fertility
Recommended Citation
Magat, Monaliza B.; Manigbas, Norvie L.; and Rey, Jessica D., "Validation of newly designed SSR markers for nine rice (Oryza sativa L.) genotypes with variable heat tolerance responses based on agromorphic data and pollen fertility analysis" (2023). Journal Article. 5882.
https://www.ukdr.uplb.edu.ph/journal-articles/5882
Identifier
https://pas.uplb.edu.ph/issues/march-2023-vol-106-no-1/
Digital Copy
Yes
En – AGROVOC descriptors
ORYZA SATIVA; GENOTYPES; HEAT TOLERANCE; POLLEN; FERTILITY; GENETIC MARKERS