Optimum conditions in the use of young inflorescence for transgenic plant regeneration of rice (Oryza sativa L.)

Issue Date

6-2001

Abstract

Current breeding in rice focuses on developing new plant type (NPT) lines with long panicles and strong and thick culms to support more seeds - characteristics that make NPT lines highly prone to the attack of pests. Genetic engineering is now geared toward improving NPT lines to increase their resistance to pests and diseases. Conditions for A. tumefaciens-mediated transformation of NPT elite lines had been optimized by considering the explant, selection conditions and the different artificial media used. Transgenic plants were obtained from calli derived from mature seeds and young inflorescence using the developed protocol. Transgenic plants were grown to maturity, and ploymerase chain reaction and Southern blot analyses confirmed the presence of the transgene in the transformed NPT lines. Ours is the first report of successful genetic transformation of young rice inflorescence.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

84

Issue

2

Page

181-191

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

illustrations; tables; references

Language

English

Subject

Oryza sativa, new plant type lines, genetic engineering, Agrobacterium tumefaciens

En – AGROVOC descriptors

RICE; ORYZA SATIVA; INFLORESCENCES; TRANSGENIC PLANTS; REGENERATION (BIOLOGICAL); PLANT BREEDING; AGROBACTERIUM TUMEFACIENS; GENETIC ENGINEERING; GENETIC TRANSFORMATION; PCR; CALLUS; HYGROMYCIN

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