Cloning of a repetitive element which distinguishes banana strains of Ralstonia solanacearum in the Philippines

Issue Date

7-1999

Abstract

A partial genomic library of a banana-infecting bugtok strain of Ralstonia solanacearum was constructed in Escherichia coli DH5-α. The library was subjected to colony blotting and probed with genomic DNA of the organism labeled with alkali-labile digoxigenin. Four recombinant clones showed strong hybridization signals and were likely to harbor repetitive elements. The plasmid DNA of these clones were isolated, labeled and used to probe the EcoRI-digested genomic DNA of this organism. Among the clones, pM114 was shown to harbor a repetitive element. The size of the insert is 2.28 Kb. When pM114 was used to probe Southern blots of EcoRI digested genomic DNA of 97 strains of the organism isolated from bugtok and moko infected banana plants collected from different areas of Visayas and Mindanao, 20-21 hybridization bands were observed. There was a low level of polymorphism, since 91 strains had the same hybridization pattern, with the remaining six differing by just the presence of an extra copy. Most of the 57 non-banana strains analyzed had 0-7 bands homologous to the repetitive element. Using pM114 as probe, the bugtok and moko banana strains cannot be differentiated from each other but are clearly distinguishable from the vegetable strains of R. solanacearum.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

82

Issue

3

Page

338-350

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

illustrations; tables; references

Language

English

Subject

cloning, bacterial wilt, Ralstonia, repetitive element

En – AGROVOC descriptors

BANANAS; MUSA (BANANAS); BANANA XANTHOMONAS WILT; RALSTONIA SOLANACEARUM; DISCOLOURATION; DNA; BLUMERIA GRAMINIS; CLONES; REPETITIVE SEQUENCES; MICROBIOLOGICAL STRAINS; BACTERIAL GENOMES; DNA HYBRIDIZATION

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