Development of a fermentation medium and detection bioassay for the local production of the antihelminthic antibiotic avermectin

Issue Date

10-2000

Abstract

Streptomyces avermitilis, bacterial producer of avermectin, the antihelminthic compound for domestic animals, grew best and produced the highest yield on Dutton's seed and other production media. Subsequently, cheap and locally available substrates were screened as substitutes for the carbon and nitrogen sources of the media. Among the substrates tested, namely, all-purpose flour, cane sugar, and cassava starch, the latter was the best substitute for soluble starch. Baker's yeast was established as a better substitute for cottonseed flour than soybean meal. Restricted random balance design experiments were performed on the modified production medium 8 (MPM 8), resulting in an optimized formulation of MPM 8 consisting of cassava starch, baker's yeast, potassium phosphate and zinc sulfate.


The bioassay for the determination of avermectin activity was established using the brine shrimp Artemia franciscana.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

83

Issue

4

Page

348-356

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

graphs ; tables ; references

Language

English

En – AGROVOC descriptors

ANTHELMINTICS; ANTIBIOTIC PROPERTIES; ANTIBIOTICS; STREPTOMYCES; AVERMECTINS; ARTEMIA FRANCISCANA; BRINE SHRIMP CULTURE; FERMENTATION; GLUTAMIC ACID; MONOSODIUM GLUTAMATE; NITROGEN CONTENT; YIELD COMPONENTS

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