Isolation and characterization of acetobacter diaztrophicus (Gillis) in Saccharum officinarum L., S. spontaneum L., and Erianthus sp.

Issue Date

4-2000

Abstract

The nitrogen-fixing endophytic Acetobacter diazotrophicus was isolated from washed roots and stems of 11 out of 25 varieties of sugarcane (Saccharum officinarum L.) grown in various locations in the Philippines. It was also isolated from the roots of sugarcane-related plants, Saccharum spontaneum L. and Erianthus sp. This is the first report of its isolation from these two grasses, increasing its known host plants from five to seven. The bacterial isolates produced indoleacetic acid and catechol-type siderophore, and their identities were confirmed by polymerase chain reaction with a species-specific oligonucleotide primer. The isolation of the organism from only the undiluted samples and our many failed attempts to detect and enumerate it culturally in diluted samples may suggest that its population in Philippine sugarcane cultivar is quite low.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

83

Issue

2

Page

173-181

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

illustrations; tables; references

Language

English

Subject

Acetobacter diazotrophicus, nitrogen-fixation, sugarcane--varieties

En – AGROVOC descriptors

SUGAR CANE; SACCHARUM OFFICINARUM; SACCHARUM SPONTANEUM; ERIANTHUS; PLANT AVAILABLE NITROGEN; BIOLOGICAL NITROGEN FIXATION; NITROGEN-FIXING BACTERIA; ENDOPHYTES; NITROGENASE; SIDEROPHORES; IAA; NUCLEIC ACIDS; CHARACTERIZATION; ISOLATION

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