Method for testing osmotic effect of salinity on leaf elongation rate (LER) of rice seedlings

Issue Date

4-2000

Abstract

A series of experiments were initially conducted to develop a method of producing optimum growth of axenic whole plants inside test tubes. LER of rice plants inside test tubes using the developed protocol was comparable to that of plants in the open bath up to the third-leaf stage. The protocol produced microorganism-free plants and enabled comparison between effects of high molecular weight sugars and NaCl for several days without contamination.

The developed method worked well in determining the osmotic effect of NaCl and showing that growth reductions during salinity can be partly explained by the osmotic effect of NaCl. At high salinity, low osmotic potential of the medium can reduce growth. Decrease in LER of seedlings grown in 300 mOs NaCl or mannitol within 5 d suggests that the osmotic effect of NaCl can last for several days. Moreover, the NaCl stressed Nona Bokra plants fully recovered within 24 h after the concentration was reduced from 300 mOs to 2 mOs and had higher LER than the control within 2 d.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

83

Issue

1

Page

125-132

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

graphs, tables, references

Language

English

Subject

axenic plants, leaf elongation rate, neutral osmotica, osmotic adjustment, salt tolerance

En – AGROVOC descriptors

ORYZA; RICE; OSMOTIC ADAPTATION; OSMOTIC STRESS; OSMOTIC POTENTIAL; CHEMICOPHYSICAL PROPERTIES; SODIUM CHLORIDE; SALINITY; SALINITY STRATIFICATION; MANNITOL; GLUCOSE; PLANT PHYSIOLOGY; PLANT GROWTH SUBSTANCES; SALT TOLERANCE; SALINE SOILS; DROUGHT STRESS; AXENIC CULTURE; HYDROCULTURE; FOLIAR NUTRITION; NUTRIENT UPTAKE

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