Murihexocin C from the Leaves of Annona squamosa Linn. Induces Apoptosis in Human Colon Carcinoma Col 2 Cell Line

Issue Date

6-2009

Abstract

The acetogenin murihexocin C was isolated from the leaves of Annona squamosa Linn. The structure was assigned based on 1H and 13C NMR and HR-ESI-TOF-MS. This is the first report of the compound from this plant. Murihexocin C exhibited significant cytotoxicity to human colon carcinoma Col 2 cells (IC 50 0.50 μg mL -1 or 0.79 μM) and normal Chinese hamster ovarian cells AA8 cells (IC 50 0.78 μg mL -1 or 1.24 μM). Murihexocin C (50 μg mL -1) induced extensive apoptotic cell death in Col 2 cells at 12 h as demonstrated by the TUNEL assay. Activation of the apoptotic effector caspase 3 was observed and a Bcl-2-like protein was noted to be down regulated.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

92

Issue

2

Page

122-132

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

illustrations; graphs; tables; references

Language

English

Subject

apoptosis, anticancer, colon cancer, cell culture, chromatography, cytotoxicity, natural products

En – AGROVOC descriptors

ANNONA SQUAMOSA; APOPTOSIS; DRUG THERAPY; CYTOTOXICITY; COLON; CELL CULTURE

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