Activity of the Extracts and Indole Alkaloids from Alstonia scholaris Against Mycobacterium tuberculosis H37Rv

Issue Date

9-2008

Abstract

The crude methanolic extract of Alstonia scholaris (L.) R. Brown demonstrated in vitro antituberculosis activity (89% inhibition against Mycobacterium tuberculosis H37Rv at 50 μg mL-1) using Microplate Alamar Blue Assay (MABA). Gradient pH fractionation of the alkaloids gave three alkaloid extracts, AsA, AsB and AsC, which exhibited 69%, 99% and 99% inhibition, respectively. Group separation by silica gel vacuum liquid chromatography (VLC) of extracts AsA and AsB afforded fractions that showed 69–99% inhibition against the test mycobacterium. The previously reported indole alkaloids - 19,20Evallesamine (1), a mixture of angustilobine B N4-oxide (2) and N4-methyl angustilobine B (3), 20Stubotaiwine (4), 6,7-seco-angustilobine B (5) and (+)-manilamine (6) from the most bioactive alkaloid fractions with 98–99% inhibition - showed weak activities. Among the six compounds, only alkaloid 4 demonstrated the highest activity with a minimum inhibitory concentration (MIC) of 100 μg mL-1. Compared with the standard rifampin (MIC 0.125 μg mL-1), all alkaloids were considered inactive.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

91

Issue

3

Page

348-351

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

illustrations; graphs; tables; references

Language

English

Subject

Alstonia scholaris, antitubercular activity, dita, indole alkaloids, Mycobacterium tuberculosis H37Rv

En – AGROVOC descriptors

ALKALOIDS; MYCOBACTERIUM TUBERCULOSIS; TUBERCULOSIS; BIOCHEMISTRY; CHEMICAL ANALYSIS

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