Serological and molecular detection of newcastle disease virus in captive psittacines in a wildlife rescue center in Luzon, Philippines

Issue Date

1-2021

Abstract

Newcastle disease (ND) is a viral infectious disease affecting wild and domestic poultry and is one of the major causes of the world's economic losses due to very high mortality. Limited information is known on the presence and distribution of Newcastle disease virus (NDV) in captive wild birds in the Philippines. The samples in this study were from captive psittacines in a wildlife rescue center in Luzon, Philippines. The birds were unvaccinated and did not show clinical signs of ND during the time of study. Thirty-four (91.89%) samples were seropositive for NDV using hemagglutination inhibition test with geometric mean titers ranging from 22 to 210.5. Reverse Transcription Polymerase Chain Reaction (RT-PCR) showed 7 (18.34%) of the pooled oropharyngeal and/or cloacal swab samples of birds belonging to Agapornis spp., Tanygnathus lucionensis, Cacatua galerita, Psittacus erithacus, and Melopsittacus undulatus were NDV positive. Presence of antibody without detectable virus suggests that the birds have already recovered from infection and may not have been shedding the virus at the time of sample collection. However, RT-PCR positive results indicates active infection during sampling. Furthermore, seropositive results in RT-PCR positive birds could mean that there is prolonged viral shedding in these species.

Source or Periodical Title

Philippine Journal of Veterinary Medicine

ISSN

0031-7705

Volume

58

Issue

1

Page

101-107

Document Type

Article

College

College of Veterinary Medicine (CVM)

Frequency

semi-annually

Physical Description

tables

Language

English

En – AGROVOC descriptors

BIRDS; NEWCASTLE DISEASE VIRUS; NEW CASTLE DISEASE; VIRAL SHEDDING; REVERSE TRANSCRIPTASE; WILDLIFE MANAGEMENT; PHILIPPINES

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