Serologic and molecular detection or porcine reproductive and respiratory syndrome virus in plasma of domestic pigs from selected backyard farms in Luzon, Philippines

Issue Date

7-2022

Abstract

As a notifiable disease in the Philippines, porcine reproductive and respiratory syndrome (PRRS) surveillance and risk assessment is necessary at the farm and regional levels. This study primarily aimed to detect anti-PRRSv antibodies using indirect enzyme-linked immunosorbent assay (ELISA) and viral nucleic acids using conventional reverse transcriptase polymerase chain reaction (RT-PCR) in plasma of domestic pigs from selected backyard farms in selected provinces in Luzon, Philippines. A total of 382 individual and 117 pooled samples were subjected to ELISA and RT-PCR, respectively. Overall, ELISA results showed 5.50% seropositivity, with Bulacan (32.14%) and Cagayan (7.25%) having the highest positivity rates. Using RT-PCR, 5.98% of pooled samples, specifically from Batangas, Marinduque, and Cagayan, tested positive for PRRS viral nucleic acids. Using binary logistic regression, respiratory symptoms were more likely to be associated with PRRS positivity in ELISA and PCR (p<0.05). The results suggest persistent PRRS challenge in provinces with positive outcomes in the assays, warranting the need for improvement in PRRS monitoring in the Philippines.

Source or Periodical Title

Philippine Journal of Veterinary Medicine

ISSN

0031-7705

Volume

59

Issue

2

Page

133-142

Document Type

Article

College

College of Veterinary Medicine (CVM)

Frequency

semi-annually

Physical Description

maps; tables

Language

English

En – AGROVOC descriptors

SWINE; PIGGERIES; PRRS; PLASMA; NIDOVIRALES; NUCLEIC ACIDS; ELISA; REVERSE TRANSCRIPTASE; PHILIPPINES

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