Analysis of Superoxide Dismutase (SOD) activity in Itik Pinas-Itim (Anas platyrhynchos L.) Semen Cryopreserved using either Glycerol or Dimethyl Sulfoxide (DMSO) as Cryoprotectant

Issue Date

1-2022

Abstract

Itik Pinas (IP), an improved Philippine native breeder duck, is gaining popularity due to a better egg laying performance. In aid of its local production and conservation, this study assessed the suitability of reported semen cryopreservation protocols through evaluation of sperm quality (i.e., plasma membrane integrity, motility, and morphology) and superoxide dismutase (SOD) activity of IP-Itim semen before and after cryopreservation using either 8% glycerol or 10% dimethyl sulfoxide (DMSO) as cryoprotectant in AU extender. Results showed that semen cryopreserved using 10% DMSO has higher post-thawing motility (M) with a lesser motility decrease (DM) compared to 8% glycerol (p = 0.0112). Meanwhile, the average SOD activity of semen cryopreserved with 10% DMSO and 8% glycerol increased from the pre-processing baseline level of 8.50 U/109 sperm to 10.45 U/109 sperm and 11.76 U/109 sperm, respectively, suggesting an increase in the oxidative stress in sperm cells during the freezing process. This study has demonstrated that 10% DMSO is a much suitable cryoprotectant than 8% glycerol for IP-Itim semen. However, testing the SOD activity from seminal plasma is recommended to acquire the total SOD activity of the cryopreserved semen and determine if SOD enzymes within the spermatozoa leak to the seminal plasma.

Source or Periodical Title

Philippine Journal of Veterinary Medicine

ISSN

0031-7705

Volume

59

Issue

1

Page

35-45

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

semi-annually

Physical Description

graphs; tables

Language

English

En – AGROVOC descriptors

ANAS PLATYRHYNCHOS; MALLARD; SUPEROXIDE DISMUTASE; SEMEN; GLYCEROL; CRYOPRESERVATION; OXIDATIVE STRESS

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