Cuticle-degrading enzyme activity of Metarhizium anisopliae (Metsch.) Sorok. isolates pathogenic to Asian Corn Borer larvae
Issue Date
4-2000
Abstract
Isolates of Metarhizium anisopliae (Metsch.) Sorok., grown in liquid medium containing Ostrinia furnacalis (Guenee) larval cuticle as sole source of carbon and nitrogen, were assayed for production of extracellular ptotease, chitinase, esterase, lipase amd presumptive chymoelastase. Correlation studies revealed inverse relationships between virulence, expressed as median lethal time in day, and protease, chitinase, esterase and presumptive chymoelastase activities. Virulence did not correlate with lipase production. Although the implication of individual enzymes in virulence is quite evident, the patterns of enzyme production, expressed as percent maximal activity, could not clearly account for the differences in virulence among the isolates. In contrast, variation in mean percent maximal activity, representing the overall enzyme production of each isolate, paralled the differing virulence among isolates, indicating that the whole cuticle-degrading enzyme machinery, rather than the individual enzymes, determines virulence.
Source or Periodical Title
Philippine Entomologist
ISSN
0048-3753
Volume
14
Issue
1
Page
15-29
Document Type
Article
Frequency
semi-annually
Physical Description
tables, graphs
Language
English
Recommended Citation
Santiago, Dante R. and Gabriel, Bernardo P., "Cuticle-degrading enzyme activity of Metarhizium anisopliae (Metsch.) Sorok. isolates pathogenic to Asian Corn Borer larvae" (2000). Journal Article. 6198.
https://www.ukdr.uplb.edu.ph/journal-articles/6198
En – AGROVOC descriptors
METARHIZIUM ANISOPLIAE; OSTRINIA FURNACALIS; ENZYME ACTIVITY; VIRULENCE; PROTEASE; CHITINASE; ESTERASES; LIPASES
