Antifungal effects of Thai medicinal plants against Collectotrichum gloeosporioides Penz.
Issue Date
9-2009
Abstract
This study was conducted to develop more efficient and environmentally sound methods for the control of anthracnose. Twelve Thai medicinal plants were tested as potential anti-fungal agents against Colletotrichum gloeosporioides Penz. Hexane and ethyl acetate extracts from the pericarp of Areca catechu and hexane extract from the flower buds of Etlingera elatior demonstrated high inhibitory activity of C. gloeosporioides mycelial growth with EC 50 values of 796.2, 803.9 and 803.9 μg mL -1, respectively. These extracts were fractionated and purified by column chromatography. Consequently, six compounds from the A. catechu high active extracts and two compounds from the E. elatior high active extract were isolated. Based on the results of structural elucidation, the six compounds obtained from the A. catechu high active extracts were fernenol, arundoin, lauric acid, myristic acid, palmitic acid and a mixure of β-sitosterol and stigmasterol. The two compounds obtained from the E. elatior high active extract were lauric acid and a mixture of p-sitosterol and stigmasterol. The effects of these compounds on spore germination, germtube elongation and appressorium formation were investigated; fernenol was found to provide the highest inhibition in all aspects based on EC 50 values of 45.8, 26.7 and 65.6 μg mL -1. Lauric acid, myristic acid and palmitic acid with EC 50 > 200 μg mL -1 had the least inhibitory activity. The results suggest that the compounds isolated from the pericarp of A. catechu and the flower buds of E. elatior may be applicable for the control of anthracnose diseases.
Source or Periodical Title
Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)
ISSN
0031-7454
Volume
92
Issue
3
Page
265-270
Document Type
Article
College
College of Agriculture and Food Science (CAFS)
Frequency
quarterly
Physical Description
tables; references
Language
English
Subject
anthracnose, antifungal, Areca, Colletotrichum, Etlingera, extract
Recommended Citation
Punnawich, Yenjit; Montree, Issarakraisila; Warin, Intana; and Kan, Chantrapromma, "Antifungal effects of Thai medicinal plants against Collectotrichum gloeosporioides Penz." (2009). Journal Article. 6267.
https://www.ukdr.uplb.edu.ph/journal-articles/6267
En – AGROVOC descriptors
GLOEOSPORIUM; COLLETOTRICHUM; ANTHRACNOSIS; ARECA CATECHU; ANTIFUNGAL PLANTS; MEDICINAL PLANTS; ANTIFUNGAL PROPERTIES