Direct and indirect in-vitro plant regeneration and antioxidant production in Tsaang Gubat [Carmona retusa (Vahl.) Masam]

Issue Date

3-2010

Abstract

In vitro plant regeneration of tsaang gubat [Carmona retusa (Vahl.) Masam] via direct and indirect methods using shoot tip explants was studied. The level of antioxidants such as tannin, flavonoids and phenolics in the leaves of in-vitro and field-grown plants was also evaluated. Multiple shoot formation was enhanced when shoot tips were cultured on Murashige and Skoog's (MS) medium containing 5 mg L(-1) 6-benzylaminopurine (BAP). Higher BAP concentration did not induce more new shoots; instead, the original shoot inoculum appeared stunted, vitrified and had abnormally large leaves. Continuous subculturing (6-8 wk) of the regenerated shoots to MS + 5 mg L(-1) BAP and 15% (v/v) coconut water induced shoot proliferation with an average of 15-20 shoots per inoculum after the 3(rd) subculture. MS medium containing 0.5 mg L(-1) naphthaleneacetic acid (NAA) and 4 mg L(-1) BAP was found optimum for callus formation. Subsequent differentiation of shoots was observed only in media containing BAP with the highest number of shoots produced at 10 mgL(-1). Regenerated shoots were successfully rooted on indole-3-butyric acid (IBA)-supplemented medium; the longest and highest number of roots was recorded at 10 mg L(-1) IBA. Levels of phenolics, flavonoids and tannins were higher in the leaves of tissue-culture-raised plants than those of in vivo grown plants. Likewise, plants maintained for long-term (24 wk) under in vitro conditions produced these secondary metabolites, although at lower levels compared with the field-grown materials.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

93

Issue

1

Page

47-55

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

illustrations; tables; references

Language

English

Subject

antioxidants, callus induction, Carmona retusa, in vitro culture, plantlet regeneration, shoot induction, tsaang gubat

En – AGROVOC descriptors

BORAGINACEAE; SHOOTS; IN VITRO REGENERATION; VITROPLANTS; MERISTEM CULTURE; ANTIOXIDANTS

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