Antioxidant Activity of a New Indole Alkaloid from Cassia alata L

Issue Date

9-2010

Abstract

Free radicals have significant effects on the structure and general function of the cell. They play a role in the body's defense against infection by microorganisms. At the same time, they cause a number of diseases by inducing damage to DNA and other important biomolecules. Hence, the search for additional free radical scavengers or antioxidants, especially from plant sources, is of prime importance. Leaf extracts from Cassia alata L. (locally known as 'akapulko'), traditionally used for the treatment of a variety of diseases, were evaluated as free radical scavengers using the diphenyl picryl hydrazyl hydrochloride (DPPH) assay. The ethyl acetate (EtOAc) fraction showed scavenging activity of 100%, comparable to gallic acid, a widely used antioxidant. Repeated column fractionation of the EtOAc fraction yielded a new indole alkaloid, 1-(4'-hydroxyphenyl)-2,4,6-trihydroxy-indole-3-carboxylic acid, which demonstrated a dose-dependent scavenging activity against DPPH with an IC 50 of 0.0311 μM ± 0.002, indicating strong antioxidant potential.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

93

Issue

3

Page

250-254

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

illustrations; graphs; tables; references

Language

English

Subject

Cassia alata L., DPPH free radical scavenging activity, 1-4'-hydroxyphenyl) 2, 4, 6-hydroxy-indole-3-carboxylic acid

En – AGROVOC descriptors

SENNA ALATA; LEAF EXTRACTS; ANTIOXIDANT PROPERTIES; INDOLES; ALKALOIDS

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