Seed viability and vigor testing of Jatropha curcas L.

Issue Date

12-2011

Abstract

Viability and vigor are physiological components of seed quality that are important not only in seed trade and crop production but also in germplasm conservation and management. The study aims to develop procedures for the optimum viability and vigor testing of Jatropha curcas L. seeds. Viability was determined through germination and tetrazolium chloride (TTZ) tests, while vigor was standardized using electrical conductivity test No dormancy was observed in J. curcas seeds. Germination is best using sterilized sand as substrate with the hilum facing sidewards. Uniform seed germination was observec within 3 d after sowing (DAS) with initial and final counts done after 7 and 10 DAS, respectively. The ombryo is the best seed tissue to use for TTZ test and the optimum soaking duration is 2 h. In cases vhere J. curcas seed quality is known, viability can be determined after 8 h using kernel soaked in water for 6 h For seeds with unknown or low quality, viability can be evaluated only after 26 h using whole seeds with seed coat cracked and soaked in water for 24 h. The optimum conditions for conductivity test for this species is 10 seeds soaked in 100 ml of distilled water for 31 h.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

36

Issue

3

Page

10-18

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Physical Description

illustrations; graphs; tables; references

Language

English

Subject

dormancy, electrical conductivity test, germination, Jatropha curcas, seed vigor, seed quality, seed viability, tetrazolium test

En – AGROVOC descriptors

JATROPHA CURCAS; SEED VIABILITY; SEED STORAGE LIFE; GERMINATION TESTS; ELECTRICAL CONDUCTIVITY

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