Induction of Immunity and Resistance to White Spot Syndrome Virus (WSSV) in Shrimp Penaeus monodon (Fabricius) by Synthetic Oligodeoxynucleotide and Bacterial DNA

Creator

• Edgar C. Amar, Southeast Asian Fisheries Development Center
• Joseph P. Faisan Jr., Southeast Asian Fisheries Development Center

Issue Date

9-2012

Abstract

Shrimps, like all invertebrates, are believed to lack true adaptive immunity, but recent evidence indicates that they can be protected against pathogenic organisms by priming their immune system with immunostimulatory substances. Here, we describe the results of an investigation aimed at strengthening shrimp immunity as a preventive strategy against white spot syndrome virus (WSSV) infection. Oligodeoxynucleotides (ODNs) with and without Cytosine-Guanine (CpG) motifs, and Vibrio harveyi genomic DNA (VHD) were administered by intramuscular injection and shrimp responses were assessed by ex vivo assays and experimental infection trials. Results showed that CpG ODN significantly increased ex vivo immunity indices such as total hemocyte count (THC), plasma agglutination titer (PAT), and hemocyte lysate agglutination titer (HLAT). VHD significantly increased immune indices such as THC, plasma total protein (PTP), and HLAT. Reverse (GpC) motifs increased THC only. At a lower viral challenge dose, both CpG and GpC motifs, and VHD, were able to reduce shrimp mortality significantly, but only CpG and VHD did so at a higher challenge dose. Strengthening shrimp immunity by the use of immunostimulatory nucleotides and bacterial genomic DNA could be a feasible preventive approach in the management of WSSV infections in shrimp.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

0031-7454

Volume

95

Issue

3

Page

267-277

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

graphs; table; references

Language

English

Subject

bacterial DNA, CpG-ODN, immunostimulants, innate immunity

Recommended Citation

Amar, Edgar C. and Faisan, Joseph P. Jr., "Induction of Immunity and Resistance to White Spot Syndrome Virus (WSSV) in Shrimp Penaeus monodon (Fabricius) by Synthetic Oligodeoxynucleotide and Bacterial DNA" (2012). Journal Article. 6409.
https://www.ukdr.uplb.edu.ph/journal-articles/6409

En – AGROVOC descriptors

WHITE SPOT SYNDROME VIRUS; PENAEUS MONODON; SHRIMP CULTURE; IMMUNOSTIMULANTS; IMMUNE RESPONSE; VIBRIO HARVEYI