Rapid In Vitro Micropropagation of Bambusa oldhamii Munro
Issue Date
3-2011
Abstract
Efficient and reproducible protocols have been developed to induce shoot multiplication from nodal cultures of aseptically raised seedlings and 1-yr-old plants of Bambusa oldhamii Munro. Nodal segments cultured on Murashige and Skoog (MS) basal liquid medium containing 3% sucrose with 4.4 µM 6-benzylaminopurine (BAP) induced maximum multiple shoots both in seedlings (14.2) and 1-yr-old explants (10.8). Addition of 40 µM adenine sulphate (AdS) with BAP showed a promotive effect on shoot multiplication in seedling explants. Regenerated shoots exhibited highest rooting percentage (85.0%) on half-strength MS basal medium with a combination of 9.84 µM indole-3-butyric acid (IBA) and 2.69 µM α-naphthaleneacetic acid (NAA). Plantlets were successfully acclimatized to greenhouse conditions. Plants derived from tissue culture were more vigorous than those derived from the traditional in vivo vegetative propagation method of air layering.
Source or Periodical Title
Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)
ISSN
0031-7454
Volume
94
Issue
1
Page
7-13
Document Type
Article
College
College of Agriculture and Food Science (CAFS)
Frequency
quarterly
Physical Description
tables, references
Language
English
Subject
bamboo, growth regulators, albinism, micropropagation
Recommended Citation
Thiruvengadam, M.; Rekha, K. T.; and Chung, I. M., "Rapid In Vitro Micropropagation of Bambusa oldhamii Munro" (2011). Journal Article. 6465.
https://www.ukdr.uplb.edu.ph/journal-articles/6465
En – AGROVOC descriptors
BAMBUSA; BAMBOO SHOOTS; IN VITRO CULTURE; PROPAGATION BY MICROCUTTINGS; REGENERATION (BIOLOGICAL); GROWTH CONTROL; ALBINISM