Morphometric and molecular characterization of root-knot nematodes infecting Malabar spinach (Basella Alba L.) in the Philippines

Abstract

© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. Root-knot nematodes (Meloidogyne spp.) are one of the most damaging plant parasites hence, accurate identification of these pests is essential for the formulation of an efficient disease management program. In the present study, Meloidogyne sp. infecting Malabar spinach in Los Banos, Laguna, Philippines was characterized based on morphology and molecular data. The perineal patterns of adult females showed a distinct arrangement typical of M. incognita. Morphometric analysis of some of the females and second-stage juveniles matched well to the reference M. incognita isolates from selected studies. In terms of molecular characterization, amplification based on the COII/16SrRNA intervening regions of the mtDNA and M. incognita-specific SCAR marker followed by sequencing revealed that the Malabar spinach isolate is indeed M. incognita. Although there are certain differences observed in some characters and morphometrics intraspecifically, both the overall morphological and molecular findings assert the species identity of the isolate.

Source or Periodical Title

Archives of Phytopathology and Plant Protection

ISSN

3235408

Page

1018-1034

Document Type

Article

Subject

Basella alba: perineal pattern, COII/16SrRNA, Meloidogyne incognita, SCAR

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