Isolation and genetic characterization of Salmonella spp. In chicken eggs from public wet markets in Metro Manila, Philippines

Abstract

© 2019, University of the Philippines at Los Banos. All rights reserved. Salmonella spp. is a major food-borne bacterial pathogen, with chicken meat and egg products being the main reservoirs of infection to humans. In this study, a total of 2,040 chicken eggs were randomly collected from the major public wet markets of the 17 municipalities and cities of Metro Manila, Philippines to determine the level and distribution of Salmonella spp. in chicken eggs in the region. Of the 102 pooled egg samples (20 eggs/pool) tested, no Salmonellae were isolated from internal egg contents while 13 pooled shell egg samples were Salmonella-positive, yielding an egg isolation rate of 1.27-25.49%. Salmonella-positive samples were obtained from 11 of the 17 public wet markets examined. Based on egg sizes, isolation rates of 0.29-5.88% (small), 2.64-52.94% (medium) and 0.88-17.65% (large) shell eggs were observed. Genetic characterization using panels of multiplex PCR assays showed that the major serogroups detected were B (23.08%), C1 (15.38%), C2 (15.38%) and E1 (7.69%). The most prevalent serovars identified were S. Mbandaka S. Braenderup, S. Anatum, S. Heidelburg, S. Paratyphi B and S. Livingstone (7.69%); and S. Newport (15.38%). Data on Salmonella contamination in the Philippines will be valuable in benchmarking studies and formulation of more efficient strategies to improve the quality and safety of local egg products for domestic consumption, food processing and exports.

Source or Periodical Title

Philippine Journal of Veterinary Medicine

ISSN

317705

Page

74-82

Document Type

Article

Subject

Chicken eggs, Metro Manila, Public market, Salmonella, Serovars

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