Antimicrobial susceptibility profiles and detection of colistin resistance mcr-1 gene in Salmonella enterica isolates from chicken eggs in Metro Manila, Philippines

Abstract

© 2019, University of the Philippines at Los Banos. All rights reserved. The antimicrobial susceptibility profiles of 13 Salmonella enterica isolated from chicken eggs from various areas of Metro Manila, Philippines were determined. Results showed that Salmonella isolates had the highest resistance to erythromycin (92.31%), doxycycline (53.85%), sulphamethoxazole–trimethoprim (53.85%), tetracycline (46.15%), streptomycin (38.46%) and spectinomycin (38.46%); and highest antibiotic susceptibilities to amikacin (92.31%), ciprofloxacin (84.62%), gentamicin (84.62%), kanamycin (84.62%), norfloxacin (84.62%), amoxicillin clavulanic acid (76.92%), neomycin (69.23%) and ofloxacin (61.54%) using the standard disk diffusion method. Multidrug resistance was observed in eight (61.54%) isolates, in which two (15.38%) isolates were resistant to at least 10 different antimicrobials and one (7.69%) isolate was resistant to at least eight different classes of antimicrobials tested in the study. Based on WHO classification system, high antimicrobial susceptibilities were observed for most of the Highest Priority Critically Important Antimicrobials (61.54%-84.62%) and High Priority Critically Important Antimicrobials (69.23%92.31%), however, low antimicrobial susceptibilities were observed for Highly Important Antimicrobials (0 to 46.15%) and Important Antimicrobials (38.46%). PCR analysis showed that all isolates were negative for the mcr-1 resistance gene. Data obtained in this study may serve as a guide for poultry clinicians and farm owners in their selection of effective antimicrobials against Salmonella and formulation of more effective control programs against Salmonella infection in layer chickens.

Source or Periodical Title

Philippine Journal of Veterinary Medicine

ISSN

317705

Page

83-91

Document Type

Article

Subject

Antimicrobial sensitivity, Chicken eggs, Mcr-1 gene, Metro Manila, Salmonella

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