Optimization of acid and steam explosion pretreatment of cogon grass for improved cellulose enzymatic saccharification

Abstract

© 2019 Eurasian Chemico-Technological Journal. Acid-impregnation and its combination with steam explosion were evaluated and optimized using Response Surface Methodology. At 10% solid-liquid ratio, cogon was impregnated with diluted H2SO4 solution (0 to 3%, w/w) at different ranges of temperature (40 to 120 °C) and varied time (0 to 130 min). Impregnated samples were then subjected to enzymatic saccharification using 60 FPU/g Accelerase 1500™. After enzymatic saccharification, the concentration of reducing sugar released was measured using colorimetry. Based on the results, Response Surface Model (RSM) showed that the optimum condition, predicting 7.18% Reducing Sugar Yield (RSY), was impregnation of cogon using 1.9% H2SO4 at 91.8 °C for 56 min. Experimental verification of optimum condition, done in triplicates, showed 6.35 ± 0.05% RSY. Acid-impregnated cogon was subjected to steam explosion to improve saccharifiability. Factors varied were temperature (137 to 222 °C) and exposure time (17 to 582 s). Steam-exploded samples were saccharified and RSY was determined. RSM indicated that the best steam explosion condition, predicting 7.91% RSY, was 179 °C and 500 s. Experimental verification of optimum condition showed 8.78 ± 0.02% RSY. Using RSY as basis, steam explosion improved saccharifiability of H2SO4-impregnated cogon by 38%, thus, increasing production of reducing sugars for potential bioethanol production.

Source or Periodical Title

Eurasian Chemico-Technological Journal

ISSN

15623920

Page

143-147

Document Type

Article

Subject

Acid-impregnation, Cogon, Lignocellulosic feedstock, Saccharification, Steam explosion

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