Digital image photometry of Xylose in liquid medium

Abstract

© 2019, Department of Science and Technology. All rights reserved. Digital image photometry (DIP) was used to determine xylose concentration in liquid medium. Xylose was quantified by its color reaction with phloroglucinol in a 96-well microplate sample holder using a commercial flat-bed scanner followed by image analysis with free-access software (ImageJ). The Red (R) values showed linear correlation versus xylose concentration in the range of 1.0–10.0 mg/mL with correlation coefficient of 0.987. The DIP method was found to be accurate based on experimental pentose (versus theoretical) concentration values. The observed limits of detection (LOD) and quantification (LOQ) for xylose were 0.65 mg/mL and 2.17 mg/mL, respectively. The DIP method gave results that are reliable and comparable to other technical protocols requiring expensive equipment such as high performance liquid chromatograph (HPLC) or UV-visible spectrophotometer. The DIP method was used to evaluate the extent of xylose assimilation in liquid fermentation medium by four yeast strains.

Source or Periodical Title

Philippine Journal of Science

ISSN

317683

Page

217-224

Document Type

Article

Subject

Digital photometry, Flatbed scanner, Image analysis, Phloroglucinol assay, Xylose determination

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