Utilization of hydrated petrifilm coupled with filtration in the detection and enumeration of escherichia coli in water samples

Abstract

© 2019, Department of Science and Technology. All rights reserved. Hydrated 3M™ Petrifilm™ E. coli/coliform (EC) plates coupled with filtration (HPECF) was found to be suitable in the analysis of 100 mL water samples, which is required in standard protocols in food and pharmaceutical industries. This was demonstrated with the use of suspensions of pure Escherichia coli and E. coli mixed with Enterobacter aerogenes and Pseudomonas aeruginosa – with 93–100% cell recovery. The results indicated that the HPECF method could be valuable in standard water quality analysis in pharmaceutical companies as it provides an acceptable and faster way to comply with the 2017 Philippine National Standards for Drinking Water (PNSDW). Likewise, HPECF results can be obtained after 48 h instead of 120 h as being currently used in the Most Probable Number (MPN) method, making HPECF three times faster than MPN. Furthermore, a simple cost-benefit analysis revealed that the use of HPECF can reduce the cost of water testing by 50% as compared to the MPN method. Consequently, the HPECF method is a more economical and faster alternative to the standard MPN method for water quality analysis for manufacturing companies.

Source or Periodical Title

Philippine Journal of Science

ISSN

317683

Page

395-399

Document Type

Article

Subject

Coli, Coliform, E, Filtration, Petrifilm, Water analysis

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